Trace the historical development of physical education Essay Example for Free

Trace the historical development of physical education Essay Trace the historical development of physical education and discuss the effects that these developments have had on your opportunities to develop performance in your chosen activities The historical development of physical education has had a significant effect on my opportunities and personal performance. I will be investigating the effect of historical development of physical education by looking at various Acts made during the past two centuries, especially in schools and the various forms of physical education lessons which have been used in the past. In the nineteenth century there were two distinct traditions in education Public Schools and State Elementary Schools. Public schools were for the gentry and were fee paying. They were usually non-local, residential and were single sex schools. The students played many games and concentrated on the characteristic of leadership and teamwork. They would regularly play these games and develop the rules which had recreational values. The facilities were good due to the fees paid. Physical Education has developed dramatically in public and private schools since. Education in this country started with private schooling for the social elite. Pupils enjoyed extensive facilities and focused on the character building aims of education, as preparation for responsibilities in later life as employers, officers, members of the clergy and so on. Competitive team games developed to serve these aims. There was always a concentration on sport rather than a physical education emphasis and this is still common today. Competitive fixtures are a recognised feature and the reputation from winning helps to distinguish different schools. During the eighteenth and nineteenth century State Elementary schools were for lower class people. They were free, cramped and had mixed sexes. The type of teaching was associated with military drills and Swedish gymnastics, and was more based on physical training. The lessons low status and there was lots of foreign influence. The students were valued for discipline and the training was to get them fit for war. They were more interested in social control values recreational and educational values were learnt later on. This is also changed dramatically over the years. State education began after the Forster Education Act 1870 which initiated compulsory schooling for all. In the state system physical education is compulsory and is a core subject of the National Curriculum. The class teacher is usually in charge, though is not usually a specialist. The content of the lessons is usually based on movement and ball skills. Learning by moving and doing is considered essential to the physical, emotional, intellectual and social education of young children. Variety is also important as concentration span is limited and stimulating activities need to be included. In addition to the curriculum, many schools also offer club activities like gymnastics, netball, soccer, country dancing, etc. Another major historical development was when a man called Thomas Arnold encouraged moral features of teamwork such as self discipline, loyalty, courage, character building qualities and leaders. By mid nineteenth century headmasters and staff in public schools started to organise sports. This helped with the development of games. There was local variation of games to schools from villages, the students played regularly and in their free time, school rules, skills and boundaries were developed, teams played competitively, the boys organised a self government, codified rules and inter-school fixtures began and there was the development of games elite. Athleticism was the physical endeavour with moral integrity. The cult of athleticism stressed the physical and social benefits of sports. Physical benefits were seen to cancel out the effects of inactive lifestyles. Sport was seen as therapeutic, invigorating and beneficial. It was a break from work. Sport could take place in a competitive situation which would help the boys to cope with winning and losing in a dignified manner. This in turn developed leadership qualities e.g. being captain was a high status office to hold and inspiring to many of the other students. The performance was seen as more important than the result. Athleticism also met middle class values of respectability and order. Values of sportsmanship, leadership and abiding by the rules. There were people who were against the idea of athleticism. They said that it lead to regimentation of boys thoughts and behaviour with destruction of individuality, and believed that students were finding it more important the their other studies. Old boys brought games from universities with codified rules and philosophy to excel at their sport. This contributed to technical development and improved standards. Sports Day was also introduced which gave a chance for old boys, parents and governors a chance to relate to exercise. Athleticism brought about an old boys/girls network, sports clubs and governing bodies became significant administrative features, officers in army and navy influential on troops, clergy influenced parishioners, teachers went back into schools, employers encouraged games in their workforce and the empire enabled these developments to be spread world wide. Overall it can be seen that athleticism was a benefit in developing physical education and it still carries on in todays sport. Female Education also helped development in physical education. In the late 1800s education for girls was very poor, pretentious and costly. Accomplishment for society was not intellectual development and competitiveness was not socially acceptable for women. Muscles bound was not good for childbearing and was a threat to the normal behaviour. Due to wealth in the 19th century there was more leisure time and the women were more hard headed, had more common sense and groups of middle class women overcame prejudice. The Schools Inquiry Commission 1868 was important, and in 1881, universities recognised the girls fulfilled degree requirements of boys. In 1898 there was the endowed Schools Act where by 80 girls were endowed. And by 1900 there were 36 public schools for girls, and by the 1918 Act gave girls the same education as boys. miss Bergman wrote the Teachers Encyclopaedia which introduced the principal games in girls school hockey, cricket, basketball and lacrosse. She also saw the benefit of tennis but felt students were already reasonable proficient from their social backgrounds. Womens athletics emerged in the last 1/4 of the nineteenth century. Physical activity developed later. The development was linked to sociological factors. When prejudicial attitudes began to change, girls began to participate in activities such as tennis, hockey, gymnastics and cricket. Social games like tennis allowed a mixture of sexes. Female participation in physical activity have helped to develop my opportunities to develop performance as tennis was one of the first sports women were allowed to play. The ladies were able to play privately away from the public gaze, and it was a game which helped to remove some of the stereotypes. They could run around becoming increasingly energetic and clothing began to be slightly less restrictive. Schools also accepted the game as it was non-contact, had rules and was acceptable to the parents. The middle classes also ensured its club development and the administrative structures. The lower classes had to wait until there was public provision, so their participation was delayed. Real tennis was similar it was the sport of the noblemen and royalty and in 1536 there were restrictive acts which forbade servants and labourers to play. This helped to retain the privileged status of the elite. The game was originally played with the hand but the development of rackets led to this being a more popular way of playing. It was a very sophisticated, exclusive game requiring expensive facilities, equipment and an understanding of the complex rules and social etiquette of the game. The Model Course was a major development of physical education. This course encouraged a policy of drills and physical training, but little recreational value. In 1902 the Model Course was instituted by Colonel Fox of the War Office. The aim was to improve the fitness of working class for military preparation, increase familiarity with combat and weapons, and improve discipline and obedience amongst the working class. The drills were done in uniform, in military rows with the students obeying commands. The problem was, they were teaching adult exercises to children. This model was not taking the needs of physical and mental development into account. There was no educative content and individualism. The teachers were not qualified and were of a lower status. Due to the problems the Board of Education established syllabus of physical training in 1904, 1909, 1919, 1927 and 1933. These stressed the physical and educative effect of sport. The physical content of the syllabus was influenced by the primary concern for medical and physiological base from which they approached the subject. Therapeutic effect, correction of posture faults, exercises to improve circulatory system were foremost in the aims. The educational aims were to develop alertness and decision making. The 1919 syllabus had consideration in loss of life in World War 1 and the flu epidemic after. The 1933 syllabus was more do to with the freedom of movement and was more decentralised. There was a recognition of the increasing rights of the working class and development of educational value of group word. Millions died in World War I, and so public schoolmen had ideals of service to the country and were enthusiastic about conflict. Following the war there were hopes of a more equal society due to massive loss of life sustained from all levels of power and responsibility of society. After World War II the Butler Education Act 1944 was introduced. This was a major social reform in Britain, it removed special privileges and ensured equality of opportunity to all. There were 146 local education authorities which provided recreational facilities, the leaving school age was 15, education in grammar schools was free and the 11 plus was introduced, new secondary schools were built, there were more mature forms of P.E. and the 1944 McNair Report gave physical education teachers the same status as other teachers. The rebuilding program after the war helped develop physical education. The facilities became more sophisticated, more physical education teachers were enlisted and there was a movement away from therapeutic and medical values and more emphasis on heuristic and guidance style of teaching. The movement approach was also introduced. Children used their initiative and learnt by discovery. Other major developments were that team games were giving way to more individual pursuits, travel was now available to all classes, the motor car enabled mobility fro even the working classes and air travel had become commonplace for sport teams which also resulted in an increase in competitions. Television had a major impact on sports, creating interest in new heroes in sports otherwise unknown to millions. National and international standards rose at the expense of school performances. Local authorities were supplying more recreational facilities such as swimming baths, adventure playgrounds etc. The development of National Parks opened up the countryside and encouraged outdoor activities. People were more free to choose their leisure pursuits than ever before. The Moving and Growing Programme was also a major development in the history of physical education. There were two publications Ministry of Education 1952 and 1953. The had influences of obstacle training from the army and movement training from centres of dance. There was also circuit training, weight training (progressive resistance training) and there was Outward Bound Schools promoting adventurous activities to develop personalities within natural environment in challenging conditions. This was more of a child centred approach, and as a result of educational thinking physical education teachers were now autonomous with personal control over the physical education syllabus. The activities included agility, playground and more major game skills, dance and movement to music, national dance and swimming. This was more exploratory, creative, individual and fun. Due to this programme many more activities have become available in schools curriculum, and these sports have become more ad venturous and our knowledge has increased over the years on these sports. The National Curriculum attempts to raise standard in education and make schools more accountable for what they teach. Physical Education is compulsory from the ages of 5-16. There are attainment targets and programmes of study. Children are required to demonstrate knowledge, skills and understanding involved in areas of various physical activities. There are four Key Stages. The Physical Education curriculum took place in August 1995 with Sport, Raining the Game. This has been outdated by Curriculum 2000 changed, which brought physical education more into line with other subjects. The advantages of the National Curriculum are that there is a uniformity of experience and the approach is co-ordinated. It gives the teachers guidance and there is a wide range of experience with pupils. However some would disagree and say that there is no allowance for regional variations and that it can limit choice for teachers. They also think that the tradition of schools is lost and that the cost of facilities and resources is greater. The National Curriculum can help in my performance as it helps me to judge how I am progressing and how I can improve. It gives me goals to work towards to achieve the next highest level. Assessments in physical education are also very common. The have aims and objectives and the achievement of the student is evaluated and progressed. There are several types of assessments longitudinal student profiles, purely quantitative data like fitness tests and generalised comments. Children need to show what they know, what they can do and understand. Written and verbal language is also important, and being able to use all of these in performance situations. The assessment evaluates the whole person and is not just physical. The ability to work in a group, individually and the ability to abide by the rules is also important. These assessments have helped develop my performance as teachers and coaches can give feedback on how to progress and due to mark schemes etc. they can judge how far I am progressing and what my aims are for the future and how I can improve further. The changes in society and education in the last 20 years have affected school sport i.e. extra-curricular opportunities, with a reduction in emphasis on the sporting elite. Extra-curricular clubs, open to all, became more acceptable. Although, many teachers continued to focus on competitive sports and extra curricular activities were affected by the teachers strikes in the early 1980s, financial cuts were felt in terms of transport, the local management of schools allowed schools to supplement their funds by selling off school fields, the increasing amount of leisure and employment opportunities from children meant they were less attracted to competing for their school team and the anti competitive lobby became more vocal. Competitive sport helped to develop children in sport. The children taking part would have a natural competitive instinct, and would be more motivated to practise. They would also enjoy the sport more and competition can raise self esteem and they can learn how to cope with failure and success. But some people espoused the theory that competition in sport was not good for childrens development, stating the continued feelings of failure can cause stress and anxiety and the need to win can encourage unsporting behaviour. Over the years people have also come to realise that sports can greatly benefit overall fitness including reducing the risk of dying prematurely, reducing the risk of dying from heart disease, reducing the risk of developing diabetes, reducing the risk of developing high blood pressure, helps reduce blood pressure in people who already have high blood pressure, reducing the risk of developing colon cancer, reducing feelings of depression and anxiety, helps control weight, helps build and maintain healthy bones, muscles, and joints, helps older adults become stronger and better able to move about without falling and promotes psychological well-being. Due to realising theses developments the government has produced better sport facilities which can also aide in our development of physical education. In conclusion it can be seen that all of these periods in history have contributed to the development of sport. They have increased the amount of opportunities that we have and the facilities have greatly increased. Due to many of the Acts and the National Curriculum we are now free to participate in a much larger variety of activities and not only do we involve ourselves in physical activities but we learn the educational side of sport. I think that through the times the physical education system has improved and now we have more freedom to do what we want. I think that it is good to have physical education compulsory to a certain age as it is an attempt to keep children informed of the benefits of health and fitness and can keep the children more lively and enthusiastic about sport.

The Motion Detector Sensor Engineering Essay

The Motion Detector Sensor Engineering Essay Home automation is a system that uses to help the resident to do the household activity or housework. These housework or household activity can be from pet feeding, plant watering, home entertainment system and so on. It can be operate either automatically or sometime control remotely which all depend to the home resident [2]. The entire system can be controlled by using from remove control, computer, internet until telephone. The home automation covered very wide of hoe system and it is also including home security system. The home security system basically can be separate to five categories [3], there are: Detection of intrusion Simulation of presence Detection of fire, gas and water Medical alert and precise Safe closing of blind For this project, only 2 categories have been discussed. There are detection of intrusion and detection of fire. And there are 4 types of intrusion which are [3]: i) Detection of movement ii) Magnetic contact of door or window iii) Glass breaking and pressure change iv) Pressure change 2.1 Part research and analysis 2.1.1 Motion detector sensor Only detection of movement has been discussed for this project. In here, the detection of movement means the movement that cause by human being and a motion detector sensor being use to detect the motion. A motion detector sensor can separate to active and passive and it can detect motion by using optics or acoustics. An active motion detector sensor is dispersing a constant field to detect motion. The field will remains static until something is enters it then disturbs the reflected pattern. Meanwhile, a passive motion sensor is waiting in standby mode in order to trigger a threshold setting when motion is detected. However, active and passive technique both have similar disadvantage. They only can sense very limited range that may weaken with distance. As the result, combination of both techniques has been used to gain greater effectiveness to detect the motion [4]. There are several types of motion detector sensor being use in the market [5] for example: i) Infrared motion detector sensor Infrared motion sensor is the most reliable and cheapest among the entire motion sensors available in the market. The concept of infrared motion sensor is using the infrared light spectrum to measure the heat of objects that pass in front of it. Then it will compare the heating level to heating level that cause by objects that behind it. The wavelength infrared wave is between 0.7 and 300 micrometers but shorter than the  wavelength  of  visible light  but longer than microwaves that cannot be detected by human senses but can felt as the heat. This infrared motion detector sensor can be fitted with different lenses to get greater distances. Basically the infrared motion detector sensor separate to 2 types only, there are passive and active infrared motion sensor [6]. The passive infrared sensors will detect electromagnetic radiated energy that generate by external sources like thermal energy emitted by people in the infrared range as figure. The thermal energy will reaches to the sensor that comprise more than one individual  sensor which made from pyroelectric materials. This sensor will generate an electrical voltage according it is heated or cooled [7]. Figure 1: The operation of passive motion infrared sensor [8] Meanwhile, the active infrared sensors will generate a multiple beam pattern of modulated infrared energy and react if it has change in the modulation of the frequency or an interruption in the received energy. The active infrared motion basically consist emitter and receiver pairs to generate IR detection zone. However, this emitter and receiver pairs need to adjust probably to achieve the best balancing between reflected IR energy and transmitted IR energy. This balance is called as the threshold level of reflected IR. If the reflected IR energy significantly above the threshold level, IR motion detector sensor will trigger on as figure: Figure 2: The operation of active motion infrared sensor [9] ii) Microwave motion detector sensor Figure 3: The operation of microwave motion sensor [10] The microwave motion detector sensor is type of active sensor that using radar call  Doppler radar to send out microwaves in order to detect and measure the movement of an object. Then it will receive the returning waves that cause by moving object to determine the distance, direction even the speed of the object. Basically the microwave motion detector has 2 types, they called bistatic and monostatic [11]. The bistatic type contains emitter and receiver which operate separately to configure the beam to fit particular situation. It has a larger detection range which up to 1500 linear feet but it cannot define the area of detection and easy to cause the error [12]. Meanwhile, the monostatic type can define detection area based on microwave beam that it emits. The detection range is smaller than bistatic which only 400 linear feet but it more efficient [12]. Although the microwave motion detector sensor cover a larger area than infrared sensor and more reliable over longer distances, but it is least popular because of their vulnerability to electrical interference and expensive cost [11]. iii) Ultrasonic motion detector sensor Figure 4: The operation of ultrasonic motion sensor [13] An ultrasonic  motion detector sensor will detect the movement by generating the sound wave in the ultrasonic frequency range around 30 kHz to 50 kHz within the limited area [14]. It will generate a cone-shaped sound wave and listen to the echoes. . The sound wave generally inaudible to humans and most animals but it does not pass through most objects. So, if no object is moving within the zone of detection, the pattern of sound wave in its environment and the time that reflected back will remain the same. However, if something moves, the microwave detector will detect and analysis the level or phase of returning sound waves then will trigger on the sensor [14]. Basically this ultrasonic motion detector sensor separated to 2 types, there are active and passive. [15] The active ultrasound motion detector sensors emit sound waves from quartz-crystal transducers then the sound wave will cover within the field of detection. The sound wave will not disrupt if there is no any movement detected. However, the sound wave will disrupt when movement detected and reflected back to receiver. The receiver will sent the signal to control unit and trigger on the sensor by analysis its sensitivity and calibration. Although the microwave motion detector sensor very efficiency in performance, but it is weak in very short of the field of detection that only detectable up to forty feet [15]. Meanwhile the passive motion detector sensors operate on the principle of sounds for example breaking glass to trigger on the sensor. These sounds produce sound waves that detected by the sensors which same concept like the active sensors. The signal will sent to control units to determine if the sound wave pattern falls within established normal parameters [15]. Table below is the summary of technologies to detect the motion: Technique Description Advantages Disadvantages 1) Infrared [6] Active: Generate multiple beam of modulated infrared energy and receive any interrupt or change of received energy Passive: detect electromagnetic radiated energy that generate by external sources like thermal energy i) Low cost ii) Easy to maintain i) Cover very small area 2) Microwave [12] Cover area with electronic field and receive any disturb or movement of electronic field i) Cover large area ii) Quick response i)High cost ii)Too sensitive 3) Ultrasonic [15] Generate sound wave and reflect the sound wave if any disturb or movement i) Cover large area ii) Quick response iii) Long lifetime i) Easy affect by environment vibration ii) High cost Table 1: Summary and comparison of 3 technologies to detect the motion 2.1.2 Heat detector sensor For another category which is detection of fire, there also have several techniques to detect it. These techniques are sensing in temperature, humidity and smoke [3] and sensing in temperature will be discussed in this project. There are few technologies in this market to sense the temperature [16] like table: Technologies Description Advantages Disadvantages 1)Thermocouple [17] Consist junction of 2 types of metal that joined together will produce voltage which varies with temperature change i) can used in wide temperature ii)Fast response i) Low accuracy ii) short life time 2) Resistance thermometer (RTD) [18] Electrical resistance of pure metal will increase with rise of temperature i) High accuracy ii) Good stability i)Slow response ii) High cost 3) Thermistor [19] Type of ceramic resistor that resistance will change with temperature changing i) Accuracy in small scale of temperature range ii) Good vibration i) Limit in small temperature range Table 2: Summary and comparison 3 of technologies to sense the temperature in the market 2.1.3 Wireless system application Short range radio application The table below show that the summary of some short range radio application and characteristics that use in the market. Application Frequency Characteristics Security system 300MHz -500MHz,800MHz,900MHZ Simplicity, easy installation Emergency medical alarms 300MHz-500MHz,800MHz Convenient carrying, long battery life, reliable Computer accessories UHF(Ultra High frequency) High data rates, vary short range, low cost RFID (Radio Frequency Identification) 100kHz to 2.4GHz Vary short range, active or passive transponder Wireless microphone and headphone VHF(Very High Frequency), UHF( Ultra High frequency) Analog high fidelity voice modulation, moderate price Keyless entry(gate opener) UHF(Ultra High Frequency) Miniature transmitter, special coding to prevent duplication Wireless bar code readers 900MHz, 2.4GHz Industrial use, spread spectrum Table 3: Short range radio application [20] Figure 5: Radio communication link diagram [21] Figure above is the radio communication link diagram. In transmitter, the source data will send to encoder to create a group of bits then sent the signal to receiver to make sure that no any false occurrences happen. Then the receiver will check and only accept the signals that the same address bits same with it. The signal that sent which also call message frame has 4 fields [21] like figure: Figure 6: Message frame [21] The first field is called preamble that as start bits to tell the receiver the message begins. Next field is called address, it will identify address and notifies the receiver come from which units that message is coming which sent by transmitter. Then the data field will follow on which it may indicate what type of event is being signaled in some protocols. Finally last field is parity which allows the receiver to determine whether the message was received correctly or not [21]. When several transmitters are used with the same receiver, all transmitters need to have the same identification address which set by receiver where receiver need to be set to recognize the identification address of all the transmitters that used in the system. Table below is the summary of advantage and disadvantages of two addressing system [21]: Advantages Disadvantages Unlimited number of transmitter can be used with a receiver Limited number of bits increase false number and interference from adjacent system Can be used with commercially available data encoder and decoders Device must be opened for coding during installation Transmitter and receiver can be easily replaced without recoding the opposite terminal Must be used with a dedicated microcontroller and cannot be used with standard encoders and decoders Each transmitter is individually recognized by receiver Limited number of transmitters can be used with each receiver Large number of code bits reduces possibility of false number Longer code reduces probability of detection Table 4: Advantages and disadvantages of two addressing system [21] 2.2 Review of similar product No Product Specification Cost Reference 1 Motion detector alarm set Detect motion and activates alarm -Build in high output 105dB alarm -Keypad to arm or disarm and security code -120VAC adapter operate US $24.99 [22] 2 Wireless motion system CWA2000 Transmitter can transmit signal to receiver until half mile away -Activates LED and buzzer on base station when detect motion -Expendable up to 8 motion sensor -Can adjust sensitivity of detection -Receiver required AC power and 4 AA batteries for backup mode -Sensor required 4 AA batteries US $99.00 [23] 3 Skylink SC-10 wireless Home security system -Consists 2 door or window sensors and 2 keychain transmitter -Arm or Disarm by using keychain transmitter -Build in high output 110dB siren -Expendable up to 15 sensors operate by AC power and 4 AA batteries for backup mode US $109.95 [24] 4 Powermax + wireless intruder alarm -Support real time viewing from camera -Consist 3 PIR sensor and 2 magnetic door sensor with wireless -External high output siren -Sent message to user by email, SMS or phone when any incident happened -Support latchkey to inform parent when child arrive to home -Remote home management which provide control and status update up to electrical devices -Required AC power to operate  £257.00 [25] 3.7 Hardware description 3.7.1PIC16F874A microcontroller Figure 14: Pin description of PIC16F874A microcontroller [26] The behavior of the Home Security system basically control by three PIC 16F8XX microcontrollers. The PIC16F8XX microcontroller is under Microchip PIC microcontroller device and very commonly being use because of low-cost, low-power consumption but high speed in performance and I/O flexibility if compare to other device and family. The microcontroller emphasizes almost same function as a microprocessor but PIC Microcontroller only operates at very low speed or frequency which only few MHz or even lower if compare to microprocessors that operate with high speed or frequency. Besides, PIC microcontroller consumes very small among of power consumption which only around miliwatts. So, PIC Microcontroller is more suitable for home security system compared to microprocessor in terms of size, cost, power consumption and the memory to control the input/output devices. They are three types of microcontroller also take into consideration, which are PIC 16F84A [27], PIC16F874A [26] and Atmel-AT89C2051 [28].The comparison was made before the final decision make. Below is the table of comparison between 3 microprocessors: Features Microchip (PIC16F84A) Microchip(PIC16F874A) Atmel(ATmega88V) Instruction 35 single word 35 single word 131 single word Operating speed 20MHz 20MHz 20MHz Flash Program Memory 1024 x 14 words 4k x 14 words 8k x 14words Data Memory(RAM) 68 x 8 bytes 192 x 8 bytes 1k bytes internal SRAM EEPROM 64 x 8 bytes 128 x 8 bytes 512 x 8 bytes I/O Port 13 33 23 Counter/timer 1 3 2 Counter bit 8 bits 16 bits 16 bits Series Interface (MSSP) -In-Circuit Serial Programmingâ„ ¢ (ICSPâ„ ¢) via two pins Synchronous Serial Port (SSP) with SPIâ„ ¢ (Master mode) I ²Câ„ ¢ (Master/Slave) Master/Slave SPI Serial Interface Byte-oriented 2-wire Serial Interface (Philips I ²C compatible) Operating Voltage 2.0V-5.5V 2.0V 5.5V 1.8V 5.5V Software MPLAB ® IDE MPLAB ICD2 MPLAB ® IDE MPLAB ICD2 ATAVRISP

Succinate Dehydrogenase Enzyme In Inner Mitochondrial Membrane Biology Essay

Succinate Dehydrogenase Enzyme In Inner Mitochondrial Membrane Biology Essay Succinate dehydrogenase (SDH) is an enzyme found in the inner mitochondrial membrane, which makes it an easy target to isolate when studying the citric acid cycle. This enzyme is responsible for catalyzing the oxidation of succinate into fumarate and can be used as a marker enzyme during the isolation of mitochondria through differential centrifugation. The isolated mitochondria can be treated with a sodium azide reagent to inhibit the mitochondrion transport of electron in the cell extract. To measure the activity of the enzyme, an artificial electron acceptor (2, 6-dichlorophenolindphenol, DCIP) is used to accept two electrons. Upon receiving electrons, the oxidized DCIP is reduced and the color of the mixture changes from blue to colorless. Spectrophotometry at the 600nm range can then be used to quantify this color change, and give an indication of the mitochondrial content of a given sample. As the The findings show that the experiment mimics Michaelis-Menten kinetic properties Enzymes are regulators of metabolic pathways that lower the activation energy in order to catalyze the acceleration of biochemical reactions [1]. Most enzymes are characterized as showing Michaelis-Menten (M-M) kinetic properties. Simply, enzymes work by binding its substrate reversibly changing its conformation to form an enzyme-substrate complex, and then detach to form free enzyme and product. If there is low substrate concentration, there will be very little enzyme activity and the rate of the reaction will slow down. If there is high substrate concentration, the enzyme will be more active and the reaction will be faster. At a certain point, if the substrate concentration is saturated, the rate of the reaction will not increase [1]. Along with the substrate concentration, these dynamics can be characterized as the M-M constant (Km) and maximum velocity (Vmax). These factors determine the initial velocity of the biochemical reaction and contribute to the understanding of the M-M e quation (in fig.1) However, when a competitive inhibitor is present, the inhibitor can bind to the active site to prevent the normal substrate from binding and forming the product. Thus, both the inhibitor and substrate compete for the active site of the enzyme, which based on the M-M equation, allows the Vmax to stays constant and the Km to change [2]. In the experiment, we will examine activity of SDH, an important component of the citric acid cycle that is responsible for catalyzing the oxidation of succinate to fumarate in the inner membrane of the mitochondria. The enzymatic activity will be determined by mitochondria fractionation from isolated cells of cauliflower by the technique of differential centrifugation. Also, we will determine the effects of enzyme concentration and competitive inhibition on the initial velocity of the reaction by adding the malonate, a classic competitive inhibitor. We will measure the reaction by blocking the electron transport with sodium azide and monitoring the reduction of the DCIP that can be followed by the change in spectrophotometry absorbance reading at 600 nm over time Since the oxidized form of the dye is blue and the reduced form is colorless, the reaction can be reestablished based on the experiment (in fig.2),. Thus, we hypothesize that the reaction will follow M-M kinetics as the abs orbance will decrease when the malonate is added Methods In isolating mitochondria, we removed with a scalpel 20 g of cauliflower from the outer 2-3 mm surface. Then, we grinded the tissue with a pestle in a chilled mortar in 40 ml of ice-cold mannitol grinding buffer for 4 min. We filtered the suspension and squeeze the solution out through four layers of cheesecloth into three chilled 15 ml centrifuge tube. Then, we centrifuged the filtrate solution at 1000 x gravity for 10 min and decanted the supernatant into a chilled 50 ml centrifuge tube. After, we re-spun the filtrate solution at 10,000 x gravity for 30 min at 0-4 °C and discarded the supernatant in the sink leaving the pellet. Then, we added 7.0 ml of icecold mannitol assay buffer to the mitochondrial pellet and scraped and mixed the mitochondrial pellet from the wall of the centrifuge tube with a spatula and vortex thoroughly to re-suspend the pellet in the assay buffer. Until needed, we transferred the mitochondrial suspension to a test tube and stored it in an ice bath. In measuring the activity of SDH, we label 10 test tubes or cuvettes as shown in table 1. We heated 0.6 ml of the ice cold mitochondria suspension in a boiling water for 5 min and placed it in an ice bath to cool. Then, we added correct volumes of azide, DCIP, malonate, and succinate to all labeled test tubes indicated in the table, covered them with Parafilm and inverted to blend the solutions. After, we add specific volume of the mitochondrial suspension to blanks 1-4 and tubes 1-4. Using a spectrophotometer set at 600nm, we blanked and took the absorbance of tubes 1-4 every two minutes until 20 minutes after the first reading. Then, we repeated again by taking the absorbance using only test tubes 5-7 for every two minutes. Results The spectrophotometer results we obtained are presented in Table 2, and shown graphically in Figure 3-7. In Table 2, the first 4 test tubes and blanks we were only able to take 3 readings and the test tubes 5-7, we were able to take 4 readings. The greatest absorbance reading was obtained for test tube 4 at 2.363 abs., which is because malonate, the competitor inhibitor, is present along with the substrate, succinate. In Tube 6, one of the lowest absorbance readings because it is a negative control and does not have any cellular suspension. This is shown experimentally when the reaction mixture will remain the color blue because with the succinate there is no reaction between the marker enzyme and the DCIP. In Table 3, we calculated the change in absorbance from tube 1-4 for every 2 minutes. We also calculated the initial velocity by dividing the change in absorbance by the elapsed time. In Figure 3, the graph shows the initial velocity depends on the enzyme concentration. When the e nzyme concentration is high, it start to rapidly decrease the initial velocity because the ratio of substrate to enzyme will be abnormally low, which will decrease the formation of product. In Figure 4, the graph represent the data in Table 2, where the initial velocity measured by elapsed time. The second highest reading was found for Tube 2 (0.987), which was also in concordance with the class results. This sample contained the heaviest constituents of the cell (mostly nuclei), as well as any unbroken whole cells that may have remained after the mechanical grinding and initial centrifugation at 600x. We found Tube 8 to have the third highest absorbance reading (0.626) and Tube 4 with the lowest (0.483). However, the sample from Tube 8 should have had a lower absorbance value than Tube 4, as was seen in the average class results displayed in Table 1. Tube 8 should contain the majority of the mitochondria (as well as some lysosomes), and Tube 4 should have any residual mitochondria and smaller organelles that did not remain in the pellet after the 12,000x centrifugation. Discussion There are a number of reasons why our findings did not match up with the expected results. Although improbable, it is possible that the 12,000x centrifugation for 30 minutes was not properly carried out, perhaps because the samples were not maintained at a consistent temperature of 0-40C. It is more likely that the re-suspension of the pellet (Tube C) with the mannitol assay buffer was not performed effectively. The pellet clumps may not have been properly dispersed, and so even though more mitochondria may have been present in Tube 8 (as they should have been), they were not free to interact with the other reagents in solution. A third reason may be that too much DCIP was added to Tube 8 (relative to Tube 4), and so there was an excess of the blue DCIP reagent in that sample (and hence a higher absorbance reading due to a lower degree of color loss). Differential centrifugation, when done correctly, is a reasonably effective method for mitochondrial isolation, although separation is achieved based only on size differences of the cell components. When dealing with small organelles, a more appropriate method to use may be sucrose gradient centrifugation, which allows for separation based on size as well as shape, especially when dealing with crude cellular extracts such as cauliflower. Cited Resources Nelson, D.L., Cox, M.M. (2007) Lehninger: Principles of Biochemistry, Fifth Edition, Freeman, New York, NY Gilbert, H.F. (2000) Basic Concepts in Biochemistry, Second Edition, McGraw Hill, New York, NY Figure 1 Figure 2 SDH-FADH2 + DCIP(blue) > SDH-FAD + DCIP (colorless) + 2H+ Table 1 Cuvette Assay Medium Azide DCIP Malonate Succinate Mitochondrial Suspension Blank 1 3.7 mL 0.5 mL - 0.5 mL 0.3 mL 1 3.2 mL 0.5 mL 0.5 mL - 0.5 mL 0.3 mL Blank 2 3.1 mL 0.5 mL - - 0.5 mL 0.9 mL 2 2.6 mL 0.5 mL 0.5 mL - 0.5 mL 0.9 mL Blank 3 3.4 mL 0.5 mL - 0.5 mL 0.6 mL 3 2.9 mL 0.5 mL 0.5 mL 0.5 mL 0.6 mL 4 2.7 mL 0.5 mL 0.5 mL 0.2 mL 0.5 mL 0.6 mL 5 3.4 mL - 0.5 mL 0.5 mL 0.6 mL 6 3.4 mL 0.5 mL 0.5 mL 0.6 mL 7 2.9 mL 0.5 mL 0.5 mL 0.5 mL 0.6 mL Table 2 Trial 1 Trail 2 Trial 3 Trial 4 Blank 1 0.04 0.003 0.006 0.008 1 1.10 1.16 1.158 1.112 Blank 2 0.03 0.001 0.004 0.003 2 0.64 0.644 0.648 0.645 Blank 3 0.06 0.002 0.008 0.005 3 0.56 0.06 0.670 0.682 4 2.36 1.85 2.221 2.223 5 0.83 0.73 0.723 0.720 6 0.76 0.73 0.734 0.725 7 0.78 0.72 0.704 0.705 Table 3 Time (min:sec) Tubes 1-4 à ¢Ã‹â€ Ã¢â‚¬  Abs (nm) Initial Velocity (à ¢Ã‹â€ Ã¢â‚¬  Abs/min) Time (min:sec) Tubes 5-7 à ¢Ã‹â€ Ã¢â‚¬  Abs (nm) Initial Velocity (à ¢Ã‹â€ Ã¢â‚¬  Abs/min) 11:10 -.5505 -.0500 9:10 .1057 .0117 13:10 -.0008 -.00062 11:10 .032 .0029 15:10 .4989 .03326 13:10 .0617 .0047 17:10 .5062 .0298 15:10 .1161 .00774 19:10 -.0533 -.0028 17:10 .0338 .00199 21:20 -.0043 -.0002 19:10 .0784 .0041 23:10 -.1095 -.0048 21:10 .1195 .0057 25:10 .142 .00568 23:10 .0428 .0019 - 25:10 .0781 .0031 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7

Macbeth :: essays research papers

Blood Different imagery are seen through out the play one of the imagery is blood.In Macbeth by Wiiliam Shakespear in every scene the image of blood changes, it changes through remores, bravery, and guilt. The characters affected by all of this is Macbeth and Lady Macbeth since they are the ones with blood in their hands. The imagery of blood seens to effected all the characters in the play. One that is affected by all of this is Lady Macbeth, when she is found sleepwalkig and talking to herself after the murder of Duncan and Banquo, " Here the smell of blood still. All the perfume of Aradia will not sweeten this little hands"(5.1.53-55) Shakespear by use of blood makes Lady Macbeth goes crazy by the actions that she has done. Makes it obvious that being evil brings unhappiness and guilt of what she has done in the past. At the beginning Lady Macbeth starts all of this by asking the spirits to, "make thick my blood"(1.5.50) She asking them to make her remorseless and wrong for the actions that is about to do. She did not have the control over har own conscience since she has remorse and guilt over her actions, but it is all ready too late since she has gone crazy and paranoid of blood being on her hands. Macbeth in the other hand has not yet gone crazy, but at the beginning the captain said, " Which smoked with bloody exection"(1.2.20) Shakespear uses blood in a whole defferent way. This time the meaning of blood is of the brave fighter that Macbeth is with the bloody sword he has of his enemies in the battle he fought. This time is shown as a action in which has nothing to do with beginning evil but as a fighter of a country. Macbeth does not shows his true self to his wife and hides in his words he speaks of guilt. "Will all great Neptune's ocean wash this blood clean for my hands?"(2.2.78-79) Macbeth wounders if he will ever forgive his own actions.At the banquet it is when Macbeth shows a little of his guilt of killing Banquo, since his ghost appears to him as a sign of guilt.Shakespear makes this imagery of blood as

Immigration of the Lao Iu Mein :: Laos Thailand

Immigration of the Lao Iu Mein I thought it would be an interesting idea to enlighten and inform people about the Lao Iu Mein and our process of immigrating to the U.S. as well as the challenges we have to overcome. I interviewed my parents, Lao Iu Mein refugees who immigrated to the United States from Thailand. Through this interview, I had a chance to hear for the first time the story of my parents' struggles and experiences as they journeyed to a place where they became "aliens" and how that place is now the place they call "home." During the 1960s and 70s, Laos became engulfed in the Vietnam War. The U.S. government also got involved by supporting the anti-Communist forces and getting the tribes in Laos to help them. The Iu Mein, as well as other minority tribes, provided the U.S. with armed manpower, intelligence, and surveillance. In 1975, the community forces rose in victory as the Iu Mein people began to escape to their homeland. My father said that the reason my family, as well as most of the Iu Mein in Laos, ran away was because they didn't want to be under the new Pathet Lao government. Escaping was not easy to accomplish. Many of my parents' friends who were caught trying to escape were taken to prisons, tortured, and most of them were killed. My parents were terrified of the Vietnamese soldiers and prayed that nothing would happen to them, their brothers, sisters, parents, and their son (my brother) who was 8 years old at the time. They had to flee during the night, pass through the jungles an d onto boats traveling across the Mekong River. When they reached Thailand, my parents and those who escaped with them were taken to refugee camps. The camps were fenced with barbed wire and guarded by armed Thai soldiers. The refugees were given a curfew that only allowed them to stay out before 10 pm. If they were caught disobeying curfew, they would be beaten and taken to jail. The camps received food and supplies that were provided by the United Nations Organization. My parents said that in the camps, their lives were still hard although they felt safer. My mother said that the one thing they worried most about was the fact that they wouldn't continue their farming to grow food, something that had been part of the daily lives before the camp.

Bringing hydrocephalus

The history of hydrocephalus dates back to the time of Hippocrates (he died c. 375) and even further to medieval times when physicians believed that it was caused by an extractable (sic) accumulation of water. Surgical evacuation of superficial fluid in hydrocephalus children was first described in detail by Balalaikas AY Zachary in 1744 (Scoff, Kramer, Hashish & Sunken, 1999).While there have been many great strides, both in research and treatment, it seems the great pool of knowledge resides not thin the medical community, but in the adult survivors of the condition. One can only wonder if this is due, at least in part, to a perception by the medical community that it is not a survivable condition. Actually the exact opposite is now true. With proper medical treatment (done in a timely manner) and LOTS of love and support from the patient's family, many hydrocephalus children can go on to live near normal lives.Introduction The modern era of hydrocephalus research began with the st udies of Dandy and Blackman in 1914 (Grittier, 2007). To this day their work is considered an unsurpassed nutrition toward the understanding of hydrocephalus. While I applaud their work, it has been ninety-nine (99) years since any work of a similar scope or caliber has been undertaken and it is beginning to show. As an example, when I was born in 1963, I showed the early stages of congenital hydrocephalus and my family was basically told â€Å"take him home to die†.Needless to say, I didn't die and have become – even according to the local medical community – one of the best sources of information in our area when it comes to hydrocephalus. Although I feel honored to eave such distinction, it's not enough – the medical community – especially pediatricians – need to possess that same knowledge and that is my purpose in writing this paper. L. 20-Year decline in the mortality rate for hydrocephalus Before getting too involved in why hydrocepha lus research needs to be brought into the 21st century, an explanation of the condition is needed.Hydrocephalus is defined as â€Å"A condition marked by an excessive accumulation of cerebration's fluid (SF) resulting in dilation of the cerebral ventricles and elevated interracial pressure; (it) ay also result in enlargement of the cranium and atrophy of the brain† (Williams, 2006). It can take one of two forms. It can be either communicating (meaning there is not visible cause for the blockage of SF) or non-communicating (meaning it results from an abnormal flow of SF in and around the brain. It is also known as pediatric hydrocephalus since it is normally present from birth. (â€Å"Nervous system diseases,† 2008). When I was born in 1963, the life expectancy for a child with this condition was approximately two (2) years and the only known treatment, placement of a shunt, was still in its infancy. Since that time, shunt surgery has been refined and could almost †“ depending on the individual patient – be considered an almost routine procedure. In an article published in the August 2005 issue of the Journal of Neurosurgery, Dry. J. H. Chi, ET. AY. Stated â€Å"Congenital hydrocephalus has an estimated population incidence of 0. To 0. 8/1,000 live births† (Chi, Fullerton & Guppy, 2005). They credit this to improvements in techniques for SF shunting; however, they state that data describing mortality from congenital hydrocephalus – or that demonstrate improvements in the mortality rate are scarce. For those not familiar with shunt implantation surgery, it involves making a small incision in both the top of the head and the abdomen (in the case of a ventricular-peritoneal (UP) shunt) to insert the shunt which drains excess fluid from the brain (Goodman, 2013).To validate their hypothesis, the doctors performed an electronic search of the National Center for Health Statistics death certificate database to identify deaths f rom 1979 to 1988 directly attributable to congenital hydrocephalus, spins biffed with hydrocephalus, and acquired hydrocephalus in all children in the United States aged one (1) day to 0 years. There were 10,406 deaths attributed to childhood hydrocephalus during the 20-year period reviewed. This translates to an overall mortality rate of 0. 71 per 100. 000 person-years.Additionally, their research showed infants had the highest mortality rate with 3,979 deaths. Overall during the period deaths from acquired hydrocephalus – meaning that it occurs after birth and is the result of a tumor, injury, or disease that blocks the uptake of SF – decreased 67. 5%, deaths from congenital hydrocephalus declined 66. 3 %, and deaths from spins biffed with hydrocephalus declined 30. 4%. What does it all mean? It means that the medical community needs to re-think their position on hydrocephalus as well as what they tell patients and their families.It means saying â€Å"Take him/her h ome to die,† is no longer an option. Does it mean re-inventing the wheel? For an older doctor it could very well mean that although, in most cases, it will not be quite so drastic. The Hydrocephalus Clinical Research Network (HCI) makes the following recommendations to its members: Reduce risk of infection associated with shunt surgery Approximately 8% – 10% of shunt operations result in an infection. A study is currently underway for a quality improvement technique that will reduce surgery- related shunt infection(s). (Woolens, 2013).The result will be a reduction in the infection rate thereby reducing the length of hospitalizing as well as patient morbidity. Management of hydrocephalus in premature children The HCI is investigating two (2) surgical procedures that are commonly used to manage IV-induced hydrocephalus in premature children. A sublease reservoir is an implant consisting of a tube which goes into the ventricle attached to a silicone bubble that sits under the skin. As fluid accumulates in the ventricle, it can be removed by a needle puncture through the skin and into the silicone bubble.The second procedure is an s sublease shunt, which is similar to the reservoir except that the silicone bubble under the skin has a reservoir has an opening in the side that allows fluid to flow out under the skin. The fluid is then absorbed into the tissues. Understanding the epidemiology and outcomes of Endoscopies Third Ventriloquist's or TV Endoscopies Third Ventriloquist's (TV) is a surgical procedure where a small perforation is made is made in the thinned floor of the third entrance allowing movement of SF out of the blocked ventricular system and into the intracellular cistern which is a normal SF space.The objective of this procedure, known as a interracial SF diversion, is to normalize pressure on the brain without using a shunt. Although TV is widely used in Europe to treat hydrocephalus, its use in North America is much less common. The H CI is working to understand when the procedure is indicated as well as its associated outcomes and possible complications. The use of TV is attractive due to the fact that infection s very rare as are other complications such as slit ventricles.Conclusion Our knowledge of hydrocephalus has come a long way since medieval times when it was believed that hydrocephalus was the result of extractable (sic) accumulation of water. It has even made significant strides since the groundbreaking work of Dandy and Blackman in 1914, but the medical community must not rest on its laurels. In order to fully bring the treatment of hydrocephalus into the 21st century, they must embrace research being done by organizations like the Hydrocephalus Clinical Research Network.I feel this increased awareness is long past due because, even though it has been almost [emphasis added] a half-century since I was born with the condition, the amount of information available to the medical community remain largely unchanged. I think that is due – at least in part – to the misconception that there is not a high survival rate, therefore, why waste the money? My research has shown that over the past twenty (20) years, the mortality rate for hydrocephalus has actually declined with 0. 71 per 100,000 errors/year (Chi, 2005).The vast majority of the deaths were the result of congenital hydrocephalus – meaning that it is present to some degree when the baby is born. Here is how the AC can help to make my â€Å"big idea† (the name given by our instructor to our project) a reality. Publish more articles about the condition, research into it, and current treatment such as shunting and Endoscopies Third Ventriloquist's. Do feature stories about both child and adult survivors because we have a story we would like the world to know about!

Pampered Child Book Review

Mamen also identifies he â€Å"symptoms† of the pampered child that mimic those of genuine emotional, behavioral, and psychiatric disorders and explains the dangers of misdiagnosis. While reading this book many things were brought to my attention, it connected to many related topics that were discussed in my child development course. Some topics that were related were; how to care and nurture children, disorders in children, and mainly how to control children's emotions.In my child development course my professor made it very clear to us that when caring for a child â€Å"parenting† is a very important factor in a child's life. It has also been suggested that parents most definitely matter. â€Å"In fact we are the most powerful instruments of change in a family. † There was only a small section the book where it talks about parenting. Which I thought was a little downfall for parents reading this book. There is a bigger section in the book about disorders. In my child development course we took quite a decent time going over several disorders.One specific disorder that was mention in the book was anxiety. When reading over this section of the book I was very connected to what I have learned in my course about anxiety. In the book, Mamen entions that there are three compents to anxiety which include; physiological which deals with your autonomic nervous system, cognitive which is through yours thoughts and behavioral which is related to avoidance. When looking back to my notes from class these 3 components are also mentioned.Mamen also talks about different types of anxiety, which was also talked about in my course. Stranger anxiety and separation anxiety are very common in infants and toddlers. Another topic in the book that I connected to my child development course was the section about temper tantrums. â€Å"Temper tantrums are not fun! As Mamen states that temper tantrums are not fun I can relate to my professor stating the same. The book mentions that there are two types of tantrums one being a temperamental and the other manipulative.Mamen states how to react to each of these tantrums. Just as I was reading I remembered my professor stating to â€Å"never give in† during a manipulative tantrum. The book states the same. Mamen states in her writing when dealing with manipulative tantrums, â€Å"that you must stay consist, if you're not consist you will never see the end to these tantrums. † One negative that I thought while reading about isorders she didn't explain how to deal with temperamental tantrums as well.She discussed the differences but when looking back to my notes, I noticed that my professor stated that you must â€Å"remove the cause† when dealing with temperamental tantrums. I thought Mamen could have had something along those lines while discussing temperamental tantrums. I thought that these two topics, anxiety and tantrums is where I felt connected to what I have learned th roughout my child development course. I believe that the book correlated very well with the information that I learned about each of these two topics.All in all, Mamen is very descriptive in her writings about each topic that she discusses. While reading I was very engaged in each of her topics. I did find that her topics about tantrums and disorders were very affected on how to cope with each of them. I would definitely rate this book as being useful. I would recommend it to any parent or professional trying to cope with â€Å"pampered child syndrome. † Mamen does an effective way on not only how to recognize this syndrome but also how to manage it.